Anticorps Recombinant de lapin anti-Cytokeratin 6B

Cytokeratin 6B Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242874F9

N° de cat : 85423-2-RR

Synonymes

KRT6B, 242874F9, CK 6B, CK5/6, CK6B

---

Galerie de données de validation

• 

  WB analysis using 85423-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85423-2-RR (KRT6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 85423-2-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 85423-2-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 85423-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 85423-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85423-2-RR (Cytokeratin 6B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85423-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85423-2-RR against Human Cytokeratin 6B were performed. The affinity constant is 4.41 nM.

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HaCaT, salive humain, salive humaine, tissu cutané de rat, tissu cutané de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85423-2-RR cible Cytokeratin 6B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cytokeratin 6B Protéine recombinante Ag11288
• Nom complet: keratin 6B
• Masse moléculaire calculée: 564 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC034535
• Symbole du gène: Cytokeratin 6B
• Identification du gène (NCBI): 3854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 6B (CK6B) is a type of intermediate filament protein primarily expressed in certain epithelial cells. Cytokeratin 6B consists of a central α-helical rod domain flanked by non-helical head and tail domains, which is a common structural feature of cytokeratins. CK6B is mainly expressed in the basal and suprabasal layers of stratified epithelia, such as the oral mucosa, esophagus, and conjunctiva. It is also found in some specialized epithelial cells like the hair follicle outer root sheath and the nail matrix. CK6B can serve as a diagnostic marker for certain pathological conditions. Its expression pattern can be used to distinguish between different types of epithelial tumors or to identify specific epithelial cell populations in histopathological studies.

{{ptg:RelatedPrimaryAntibodies}}