Anticorps Polyclonal de lapin anti-Cytokeratin 7-specific

Cytokeratin 7-specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16857-1-PBS

Synonymes

Cytokeratin 7, KRT7, CK 7, CK7, CK-7

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Galerie de données de validation

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  WB analysis of HepG2 using 16857-1-AP (same clone as 16857-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16857-1-AP (KRT7-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 16857-1-AP (same clone as 16857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16857-1-AP (same clone as 16857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 16857-1-AP (same clone as 16857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 16857-1-AP (same clone as 16857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16857-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16857-1-PBS cible Cytokeratin 7-specific dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 7
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: NM_005556
• Symbole du gène: Cytokeratin 7
• Identification du gène (NCBI): 3855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

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