Anticorps Monoclonal anti-Cytokeratin 8

Cytokeratin 8 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F2H5

N° de cat : 68627-1-PBS

Synonymes

KRT8, Cytokeratin,Keratin, CK-8, CK8, CK 8

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Galerie de données de validation

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  WB analysis using 68627-1-Ig (same clone as 68627-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68627-1-Ig (same clone as 68627-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  WB analysis of rat colon using 68627-1-Ig (same clone as 68627-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 68627-1-Ig (same clone as 68627-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51080-1

  Cytometric bead array standard curve of MP51080-1, Cytokeratin 8 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68627-1-PBS. Detection antibody: 68627-2-PBS. Standard:Ag0488. Range: 0.098-100 ng/mL.

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Informations sur le produit

68627-1-PBS cible Cytokeratin 8 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 8 Protéine recombinante Ag0488
• Nom complet: keratin 8
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 50-52 kDa
• Numéro d’acquisition GenBank: BC000654
• Symbole du gène: Cytokeratin 8
• Identification du gène (NCBI): 3856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

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