Anticorps Recombinant de lapin anti-Cytokeratin 8

Cytokeratin 8 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

251045E11

N° de cat : 86457-4-RR

Synonymes

KRT8, CARD2, CK 8, CK8, CK-8

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Galerie de données de validation

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  WB analysis using 86457-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsil using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsil using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human Kidney(renal cell) Cancer using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human Kidney(renal cell) Cancer using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 86457-4-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86457-4-RR (Cytokeratin 8 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 86457-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 8 antibody (86457-4-RR, Clone: 251045E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 86457-4-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 8 antibody (86457-4-RR, Clone: 251045E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 86457-4-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 8 antibody (86457-4-RR, Clone: 251045E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IHC: tissu amygdalien humain, tissu de cancer du sein humain, tissu hépatique de rat, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du sein humain,
• Résultats positifs en IF/ICC: cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86457-4-RR cible Cytokeratin 8 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 8
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC000654
• Symbole du gène: Cytokeratin 8
• Identification du gène (NCBI): 3856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 8 (CK8) is a protein that belongs to the cytokeratin family, which is a group of structural proteins found in the intracytoplasmic cytoskeleton of epithelial cells. CK8 forms intermediate filaments within the cytoplasm of simple epithelial cells, typically as a dimer with cytokeratin 18 (CK18). These filaments provide a stabilizing framework that helps determine cell shape and enables cells to cope with mechanical stress. CK8/CK18 filaments also serve as a mesh of "paths" for signaling molecules, metabolites, and pathogens to travel within the cell in an organized manner. CK8 is expressed in various epithelial tissues and is particularly associated with glandular and simple epithelia. Its expression is also a significant marker in several types of cancer, including lung and esophageal cancer, where it can indicate tumor progression and poor prognosis.

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