Anticorps Recombinant de lapin anti-Cytokeratin 80

Cytokeratin 80 Recombinant Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250883H5

N° de cat : 86313-1-PBS

Synonymes

KRT80, CK 80, CK-80, Cytokeratin-80, K80

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Galerie de données de validation

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  WB analysis using 86313-1-RR (same clone as 86313-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86313-1-RR (Cytokeratin 80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 86313-1-RR (same clone as 86313-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skin using 86313-1-RR (same clone as 86313-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 86313-1-RR (same clone as 86313-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 86313-1-RR (same clone as 86313-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86313-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86313-1-PBS cible Cytokeratin 80 dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 80
• Masse moléculaire calculée: 422 aa, 47 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC065180
• Symbole du gène: KRT80
• Identification du gène (NCBI): 144501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.

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