Anticorps Recombinant de lapin anti-Cytokeratin 80

Cytokeratin 80 Recombinant Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

250883H5

N° de cat : 86313-1-RR

Synonymes

KRT80, CK 80, CK-80, Cytokeratin-80, K80

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Galerie de données de validation

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  WB analysis using 86313-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86313-1-RR (Cytokeratin 80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse skin using 86313-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skin using 86313-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 86313-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 86313-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IF-P: tissu cutané de souris, tissu de cancer du sein humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86313-1-RR cible Cytokeratin 80 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: keratin 80
• Masse moléculaire calculée: 422 aa, 47 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC065180
• Symbole du gène: KRT80
• Identification du gène (NCBI): 144501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.

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