Anticorps Monoclonal anti-D2HGDH

D2HGDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E9B3

N° de cat : 66364-1-PBS

Synonymes

EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD, 1E9B3

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 66364-1-Ig (same clone as 66364-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 66364-1-Ig (same clone as 66364-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 66364-1-Ig (same clone as 66364-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66364-1-Ig (same clone as 66364-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66364-1-Ig (same clone as 66364-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human D2HGDH (66364-1-Ig, Clone:1E9B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66364-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66364-1-PBS cible D2HGDH dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: D2HGDH Protéine recombinante Ag5036
• Nom complet: D-2-hydroxyglutarate dehydrogenase
• Masse moléculaire calculée: 521 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC036604
• Symbole du gène: D2HGDH
• Identification du gène (NCBI): 728294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).

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