Anticorps Polyclonal de lapin anti-DAAM2

• Phare
• Validé par KD/KO

DAAM2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 25206-1-AP

Synonymes

DAAM2, KIAA0381

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Galerie de données de validation

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  WB analysis of mouse brain using 25206-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25206-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 25206-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 25206-1-AP (DAAM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 25206-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25206-1-AP (DAAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 25206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DAAM2 antibody (25206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293 transfectées, tissu cérébral de rat
• Résultats positifs en IHC: tissu cardiaque de souris, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Informations sur le produit

25206-1-AP cible DAAM2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DAAM2 Protéine recombinante Ag18955
• Nom complet: dishevelled associated activator of morphogenesis 2
• Masse moléculaire calculée: 1068 aa, 123 kDa
• Poids moléculaire observé: 100-130 kDa
• Numéro d’acquisition GenBank: BC128388
• Symbole du gène: DAAM2
• Identification du gène (NCBI): 23500
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Front Mol Biosci; Dishevelled-Associated Activator of Morphogenesis 2 (DAAM2) Predicts the Immuno-Hot Phenotype in Pancreatic Adenocarcinoma.; Authors - Qinglin Zhang
human	IHC,WB	Clin Transl Oncol; The prognostic value of DAAM2 in lower grade glioma, liver cancer, and breast cancer; Authors - Zeying Li ; KD Validated
mouse	IHC	Clin Immunol; Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy; Authors - Jie Mei

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Erithelgi (Verified Customer) (11-11-2019)

The antibody was verified in an siRNA mediated knockdown

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS osteosarcoma cells