Anticorps Recombinant de lapin anti-DAB2

DAB2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250409B4

N° de cat : 86141-1-RR

Synonymes

Adaptor molecule disabled-2, Differentially expressed in ovarian carcinoma 2, Differentially-expressed protein 2, Disabled homolog 2, DOC 2

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Galerie de données de validation

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  WB analysis using 86141-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 86141-1-RR

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 86141-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using DAB2 antibody (86141-1-RR, Clone: 250409B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86141-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86141-1-RR against Human DAB2 were performed. The affinity constant is 3.07 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Caco-2, cellules RAW 264.7
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86141-1-RR cible DAB2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DAB2 Protéine recombinante Ag0162
• Nom complet: disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 96 kDa
• Numéro d’acquisition GenBank: BC003064
• Symbole du gène: DAB2
• Identification du gène (NCBI): 1601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DAB2 is a protein of 770 amino acid residues with a predicted molecular weight of 85.5kDa. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta). Human DAB2 has an overall 83% identify with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain (PID), and contains multiple SH3 binding motifs. Chromosomal localization by FISH showed that the DAB2 gene is located on 5p13. The expression of DAB2 is down-regulated or absent in all the carcinoma cell lines examined, including prostate and ovarian carcinoma cell lines. The N-terminal domain of DAB2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of DAB2 attenuates canonical Wnt/catenin-mediated signaling, including accumulation of catenin and cyclin D1 induction. DAB2 suppresses both protein kinase C and peptide growth factor-elicited signal pathways via the Ras-mitogen-activated protein kinase pathway. The proline-rich domain of DAB2 also interacts with proteins containing SH3 domain, such as Src and Fgr. The binding of DAB2 to c-Src resultes in the inactivation of c-Src. All data suggest that DAB2 is a potent tumor suppressor in many cancer types.

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