Anticorps Polyclonal de lapin anti-DAB2IP

DAB2IP Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 30020-1-AP

Synonymes

DAB2 interacting protein, ASK-interacting protein 1, ASK interacting protein 1, AIP-1, AIP1

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Galerie de données de validation

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  WB analysis using 30020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30020-1-AP (DAB2IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of 4T1 using 30020-1-AP

  IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 2800 ug.

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  IP experiment of 4T1 using 30020-1-AP

  IP result of anti-DAB2IP (IP:30020-1-AP, 4ug; Detection:30020-1-AP 1:6000) with 4T1 cells lysate 4800 ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2, tissu cérébral de rat
• Résultats positifs en IP: cellules 4T1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30020-1-AP cible DAB2IP dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DAB2IP Protéine recombinante Ag31340
• Nom complet: DAB2 interacting protein
• Masse moléculaire calculée: 1189 aa, 132 kDa
• Poids moléculaire observé: 125-132 kDa
• Numéro d’acquisition GenBank: BC146762
• Symbole du gène: DAB2IP
• Identification du gène (NCBI): 153090
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Disabled homolog 2-interacting protein (DAB2IP) is also named as ASK-interacting protein 1 (AIP-1). DAB2IP, is a new member of the Ras GTPase-activating protein family. It interacts directly with DAB2 and has distinct cellular functions such as modulating different signal cascades associated with cell proliferation, survival, apoptosis and metastasis. The aberrant alteration of DAB2IP in cancer is caused by a variety of mechanisms, including the aberrant promoter methylation, histone deacetylation, and others (PMID: 26658103). DAB2IP is reported to bind with Apoptosis signal-regulating kinase 1 (ASK1), and is called ASK1 Interacting Protein (AIP1) (PMID: 12813029). DAB2IP function is that of a tumor suppressor in tumor cell growth, metastasis, and other aspects in cancer progression. In prostate cancer, DAB2IP expression was repressed by promoter methylation and histone modification (PMID: 12446720, PMID: 15817459).

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