- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-DACH1
DACH1 Monoclonal Antibody for IF-P
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, rat, souris
Applications
IF-P
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3B6D2
N° de cat : CL488-60082
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en IF-P | tissu cérébral de souris, |
Dilution recommandée
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
CL488-60082 cible DACH1 dans les applications de IF-P et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | DACH1 Protéine recombinante Ag4474 |
Nom complet | dachshund homolog 1 (Drosophila) |
Masse moléculaire calculée | 79 kDa |
Numéro d’acquisition GenBank | BC021219 |
Symbole du gène | DACH1 |
Identification du gène (NCBI) | 1602 |
Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells. Dach1 expression is enriched in rECs, which are associated with osteoprogenitors and bone-resorbing osteoclasts, and overexpression of DACH1 in postnatal mice induces a strong increase in arteries and type R capillaries, leading to local metabolic changes and enabling trabecular bone formation in normally highly hypoxic areas of the diaphysis (PMID: 39528700). Moreover, Loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protocole
Product Specific Protocols | |
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IF protocol for CL Plus 488 DACH1 antibody CL488-60082 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |