Anticorps Polyclonal de lapin anti-DACH1

• Phare
• Validé par KD/KO

DACH1 Polyclonal Antibody for IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC

Conjugaison

CoraLite®594 Fluorescent Dye

N° de cat : CL594-10914

Synonymes

Dachshund-1, DACH

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Galerie de données de validation

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  IF Staining of HEK-293 using CL594-10914

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 DACH1 antibody (CL594-10914) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-10914 cible DACH1 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DACH1 Protéine recombinante Ag1354
• Nom complet: dachshund homolog 1 (Drosophila)
• Masse moléculaire calculée: 79 kDa
• Numéro d’acquisition GenBank: BC021219
• Symbole du gène: DACH1
• Identification du gène (NCBI): 1602
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells. Dach1 expression is enriched in rECs, which are associated with osteoprogenitors and bone-resorbing osteoclasts, and overexpression of DACH1 in postnatal mice induces a strong increase in arteries and type R capillaries, leading to local metabolic changes and enabling trabecular bone formation in normally highly hypoxic areas of the diaphysis (PMID: 39528700). Moreover, Loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

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