Anticorps Monoclonal anti-Dystroglycan

• Phare
• Validé par KD/KO

Dystroglycan Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2B1G12

N° de cat : 66735-1-Ig

Synonymes

156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan

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Galerie de données de validation

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  WB analysis using 66735-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66735-1-Ig (Dystroglycan antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 66735-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 66735-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66735-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66735-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 66735-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 66735-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 66735-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 66735-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66735-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Dystroglycan antibody (66735-1-Ig, Clone: 2B1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66735-1-Ig

  1X10^6 HeLa cells were stained with 0.20ug Dystroglycan antibody (66735-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules NCI-H1299, cerveau de souris, tissu cérébral de porc, tissu cérébral de rat
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

66735-1-Ig cible Dystroglycan dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Dystroglycan Protéine recombinante Ag27222
• Nom complet: dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 43 kDa, 30 kDa
• Numéro d’acquisition GenBank: BC012740
• Symbole du gène: DAG1
• Identification du gène (NCBI): 1605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).

Publications

Species	Application	Title
human	FC	Proc Natl Acad Sci U S A; CACNA1S haploinsufficiency confers resistance to New World arenavirus infection.; Authors - Nicolás Sarute