Anticorps Polyclonal de lapin anti-DAK

• Phare
• Validé par KD/KO

DAK Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 12224-1-AP

Synonymes

FAD-AMP lyase, DHA kinase, cyclizing, cyclic FMN forming, Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase

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Galerie de données de validation

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  WB analysis of HeLa using 12224-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12224-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12224-1-AP

  WB result of DAK antibody (12224-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAK transfected HeLa cells.

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  WB analysis of mouse uterus using 12224-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12224-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12224-1-AP

  IP result of anti-DAK (IP:12224-1-AP, 3ug; Detection:12224-1-AP 1:500) with HeLa cells lysate 2150ug.

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  IHC staining of human stomach using 12224-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12224-1-AP (DAK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, tissu utérin de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12224-1-AP cible DAK dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DAK Protéine recombinante Ag2861
• Nom complet: dihydroxyacetone kinase 2 homolog (S. cerevisiae)
• Masse moléculaire calculée: 575 aa, 59 kDa
• Poids moléculaire observé: 55-59 kDa
• Numéro d’acquisition GenBank: BC001341
• Symbole du gène: DAK
• Identification du gène (NCBI): 26007
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DAK, also name as TKFC, Triokinase, and FMN cyclase, catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. DAK has some isoforms with MW 51-55 kDa and 59 kDa.

Publications

Species	Application	Title
mouse	WB	Endocrinology; Hepatic but not intestinal FBP1 is required for fructose metabolism and tolerance; Authors - Qiufang Bai
human	WB,IF	iScience; CircSOBP suppresses the progression of glioma by disrupting glycolysis and promoting the MDA5-mediated immune response; Authors - Maolin Mu ; KO Validated
rat	WB	Mol Psychiatry; Proteasome-independent K63 polyubiquitination selectively regulates ATP levels and proteasome activity during fear memory formation in the female amygdala; Authors - Kayla Farrell
human	WB	Pharmaceuticals (Basel); Magnolol Inhibits High Fructose-Induced Podocyte Inflammation via Downregulation of TKFC/Sp1/HDAC4/Notch1 Activation; Authors - Ziang Zhou

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