Anticorps Polyclonal de lapin anti-DAPK1

• Phare
• Validé par KD/KO

DAPK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 25136-1-AP

Synonymes

DAP kinase 1, DAPK, Death-associated protein kinase 1, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis of A549 using 25136-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25136-1-AP (DAPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 25136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25136-1-AP (DAPK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 25136-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25136-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 25136-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25136-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A549 using 25136-1-AP

  Immunofluorescent analysis of A549 cells using 25136-1-AP (DAPK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: Calu-1 cells, cellules A549, cellules MG U-87, cellules U-251
• Résultats positifs en IHC: tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

25136-1-AP cible DAPK1 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DAPK1 Protéine recombinante Ag18387
• Nom complet: death-associated protein kinase 1
• Masse moléculaire calculée: 1430 aa, 160 kDa
• Poids moléculaire observé: 140-160 kDa
• Numéro d’acquisition GenBank: BC113660
• Symbole du gène: DAPK1
• Identification du gène (NCBI): 1612
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Life Sci; Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage; Authors - Bang-Chuan Hu
human	WB,IHC,IF	Aging (Albany NY); Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma.; Authors - Zhengshuai Song
human	WB	Stem Cell Res Ther; Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.; Authors - Mengqi Chu ; KD Validated
mouse	WB	J Biol Chem; PMID: 28174303; Authors - Barbara Kroczynska
mouse	WB	J Alzheimers Dis; Oxytocin Alleviates MPTP-Induced Neurotoxicity in Mice by Targeting MicroRNA-26a/Death-Associated Protein Kinase 1 Pathway.; Authors - Hasan A M M Almansoub
human	WB,IHC	Stem Cells Dev; Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.; Authors - Shaojun Nong ; KD Validated

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