Anticorps Polyclonal de lapin anti-DAPK2-Specific

• Phare
• Validé par KD/KO

DAPK2-Specific Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 20048-1-AP

Synonymes

DAP kinase 2, DAP kinase related protein 1, DAPK2, DAPK2-Specific, DRP 1

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Galerie de données de validation

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  WB analysis using 20048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20048-1-AP

  WB result of DAPK2-Specific antibody (20048-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAPK2-Specific transfected HeLa cells.

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  WB analysis of A431 using 20048-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20048-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 20048-1-AP

  Immunofluorescent analysis of A375 cells using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HepG2
• Résultats positifs en IF: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20048-1-AP cible DAPK2-Specific dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: death-associated protein kinase 2
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 38-43 kDa
• Numéro d’acquisition GenBank: NM_014326
• Symbole du gène: DAPK2
• Identification du gène (NCBI): 23604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.