Anticorps Polyclonal de lapin anti-DAPK2-Specific

• Phare
• Validé par KD/KO

DAPK2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20048-1-PBS

Synonymes

DAPK2, DAP kinase 2, DAP kinase related protein 1, DAP-kinase-related protein 1, Death-associated protein kinase 2

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Galerie de données de validation

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  WB analysis using 20048-1-AP (same clone as 20048-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20048-1-AP (same clone as 20048-1-PBS)

  WB result of DAPK2-Specific antibody (20048-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAPK2-Specific transfected HeLa cells. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 20048-1-AP (same clone as 20048-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 20048-1-AP (same clone as 20048-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 20048-1-AP (same clone as 20048-1-PBS)

  Immunofluorescent analysis of A375 cells using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20048-1-PBS cible DAPK2-Specific dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: death-associated protein kinase 2
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 38-43 kDa
• Numéro d’acquisition GenBank: NM_014326
• Symbole du gène: DAPK2
• Identification du gène (NCBI): 23604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.

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