Anticorps Polyclonal de lapin anti-DAPK3

DAPK3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30656-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30656-1-AP (same clone as 30656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30656-1-AP (DAPK3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 30656-1-AP (same clone as 30656-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAPK3 antibody (30656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30656-1-PBS cible DAPK3 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DAPK3 Protéine recombinante Ag33051
• Nom complet: death-associated protein kinase 3
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 37, 52 kDa
• Numéro d’acquisition GenBank: NM_001348
• Symbole du gène: DAPK3
• Identification du gène (NCBI): 1613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DAPK3, also named as ZIPK and Dlk, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. The calculated molecular weight of DAPK3 is 52 kDa. This antibody can recognize the 37 kDa isoform of target. ZIP kinase is a Serine/threonine kinase which acts as a positive regulator of apoptosis. ZIP kinasephosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. ZIP kinase regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.

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