Anticorps Recombinant de lapin anti-DAPP1

DAPP1 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242582D1

N° de cat : 85346-1-PBS

Synonymes

BAM32, B-cell adapter molecule of 32 kDa, DAPP 1, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, hDAPP1

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Galerie de données de validation

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  WB analysis using 85346-1-RR (same clone as 85346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85346-1-RR (DAPP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. Jurkat cells as negative control. This data was developed using the same antibody clone with 85346-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85346-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85346-1-RR against Human DAPP1 were performed. The affinity constant is 8.12 nM.

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Informations sur le produit

85346-1-PBS cible DAPP1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DAPP1 Protéine recombinante Ag6459
• Nom complet: dual adaptor of phosphotyrosine and 3-phosphoinositides
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC012924
• Symbole du gène: DAPP1
• Identification du gène (NCBI): 27071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DAPP1 was identified as dual adaptor for phosphotyrosine and 3-phosphoinositides, a novel 280 amino acid protein which contains a putative myristoylation site at its N-terminus followed by a SH2 and a PH domain at its C-terminus (PMID: 10432293). DAPP1 is also referred to B cell adaptor molecule of 32 kDa (Bam32), and is expressed by B lymphocytes, but not T lymphocytes or nonhematopoietic cells. DAPP1/Bam32 was shown to regulate BCR signaling downstream of phosphatidylinositol 3-kinase (PI3K) (PMID: 10770799). Furthermore, DAPP1/Bam32 may server to integrate PI3K and Src kinase signaling to promote Rac-dependent B cell adhesive interactions important for antigen presentation function (PMID: 20495066).

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