Anticorps Recombinant de lapin anti-DAPP1

DAPP1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242582D1

N° de cat : 85346-1-RR

Synonymes

BAM32, B-cell adapter molecule of 32 kDa, DAPP 1, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, hDAPP1

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Galerie de données de validation

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  WB analysis using 85346-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85346-1-RR (DAPP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. Jurkat cells as negative control.

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  Affinity and Kinetic Characterization of 85346-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85346-1-RR against Human DAPP1 were performed. The affinity constant is 8.12 nM.

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Ramos

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85346-1-RR cible DAPP1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DAPP1 Protéine recombinante Ag6459
• Nom complet: dual adaptor of phosphotyrosine and 3-phosphoinositides
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC012924
• Symbole du gène: DAPP1
• Identification du gène (NCBI): 27071
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DAPP1 was identified as dual adaptor for phosphotyrosine and 3-phosphoinositides, a novel 280 amino acid protein which contains a putative myristoylation site at its N-terminus followed by a SH2 and a PH domain at its C-terminus (PMID: 10432293). DAPP1 is also referred to B cell adaptor molecule of 32 kDa (Bam32), and is expressed by B lymphocytes, but not T lymphocytes or nonhematopoietic cells. DAPP1/Bam32 was shown to regulate BCR signaling downstream of phosphatidylinositol 3-kinase (PI3K) (PMID: 10770799). Furthermore, DAPP1/Bam32 may server to integrate PI3K and Src kinase signaling to promote Rac-dependent B cell adhesive interactions important for antigen presentation function (PMID: 20495066).

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