Anticorps Recombinant de lapin anti-DARS2

DARS2 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243050E5

N° de cat : 85691-2-PBS

Synonymes

Aspartate  tRNA ligase, Aspartate--tRNA ligase, mitochondrial, Aspartyl-tRNA synthetase, AspRS, EC:6.1.1.12

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Galerie de données de validation

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  WB analysis using 85691-2-RR (same clone as 85691-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 85691-2-RR (same clone as 85691-2-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 85691-2-RR (same clone as 85691-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 85691-2-RR (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 85691-2-RR (same clone as 85691-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DARS2 antibody (85691-2-RR, Clone: 243050E5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85691-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02026-2

  Cytometric bead array standard curve of MP02026-2, DARS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85691-3-PBS. Detection antibody: 85691-2-PBS. Standard: Ag4809. Range: 0.781-100 ng/mL.

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Informations sur le produit

85691-2-PBS cible DARS2 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DARS2 Protéine recombinante Ag4809
• Nom complet: aspartyl-tRNA synthetase 2, mitochondrial
• Masse moléculaire calculée: 645 aa, 74 kDa
• Poids moléculaire observé: 66-74 kDa
• Numéro d’acquisition GenBank: BC045173
• Symbole du gène: DARS2
• Identification du gène (NCBI): 55157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).

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