Anticorps Polyclonal de lapin anti-Dopamine Transporter/DAT

Dopamine Transporter/DAT Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22524-1-PBS

Synonymes

2, SLC6A3, DA transporter, DAT, DAT1

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Galerie de données de validation

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  WB analysis using 22524-1-AP (same clone as 22524-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  IP result of anti-Dopamine Transporter/DAT (IP:22524-1-AP, 4ug; Detection:22524-1-AP 1:1000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Dopamine Transporter/DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22524-1-PBS cible Dopamine Transporter/DAT dans les applications de WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Dopamine Transporter/DAT Protéine recombinante Ag18297
• Nom complet: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• Masse moléculaire calculée: 620 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC133003
• Symbole du gène: DAT
• Identification du gène (NCBI): 6531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).

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