Anticorps Polyclonal de lapin anti-DAXX

DAXX Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (2)

N° de cat : 20489-1-AP

Synonymes

ETS1 associated protein 1, EAP1, Death domain-associated protein 6, Death domain associated protein 6, DAP6

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Galerie de données de validation

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  WB analysis of Y79 using 20489-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20489-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20489-1-AP (DAXX antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules HeLa
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

20489-1-AP cible DAXX dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DAXX Protéine recombinante Ag14519
• Nom complet: death-domain associated protein
• Masse moléculaire calculée: 740 aa, 81 kDa
• Poids moléculaire observé: 120 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC109074
• Symbole du gène: DAXX
• Identification du gène (NCBI): 1616
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Death associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, development and transcription. It has been shown to translocate from the nucleus to the cytoplasm under conditions of stress and to activate the Jun N-terminal kinase (JNK) pathway. Somatic mutations of death associated protein (DAXX) have been found in ALT cancers, including pancreatic neuroendocrine (panNET) cancers and glioblastoma multiforme (GBM) cancers.

Publications

Species	Application	Title
Mouse	WB	Cell Res; Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models.; Authors - Meng Yu
human	IF	Nat Commun; Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.; Authors - Alice Mac Kain
mouse	WB	J Biol Chem; The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.; Authors - Nasim A Begum
		Sci Bull (Beijing); P53 upregulation by USP7-engaging molecular glues; Authors - Zhaoyang Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

The antibody strongly detected DAXX by western-blot (was used as a positive control in co-immunoprecipitation experiments) but also provided many other bands in HeLa cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

Alexandros (Verified Customer) (03-03-2023)

Very happy with the final image we have in our blots compared to other antibodies we have previously used

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Adherent cells

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