Anticorps Recombinant de lapin anti-DAZL

DAZL Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251035E11

N° de cat : 86344-3-PBS

Synonymes

DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like

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Galerie de données de validation

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  WB analysis using 86344-3-RR (same clone as 86344-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86344-3-RR (DAZL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 86344-3-RR (same clone as 86344-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (86344-3-RR, Clone: 251035E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86344-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86344-3-RR against Human DAZL were performed. The affinity constant is 2.52 nM.

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Informations sur le produit

86344-3-PBS cible DAZL dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DAZL Protéine recombinante Ag37074
• Nom complet: deleted in azoospermia-like
• Masse moléculaire calculée: 295 aa, 33 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC027595
• Symbole du gène: DAZL
• Identification du gène (NCBI): 1618
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DAZL proteins are germ cell-specific RNA-binding proteins and are considered major regulators of spermatogenesis. Because DAZL is a novel protein expressed specifically in germ cells, it is widely employed as a germ cell marker. In humans, immunostaining shows DAZL is abundant in the cytoplasm of primary spermatocytes, but scarce in spermatogonia.

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