Anticorps Polyclonal de lapin anti-DBR1

• Phare
• Validé par KD/KO

DBR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16019-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human brain using 16019-1-AP (same clone as 16019-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

  WB result of DBR1 antibody (16019-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16019-1-AP (same clone as 16019-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

  IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:800) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 16019-1-AP (same clone as 16019-1-PBS)

  IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:500) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 16019-1-AP (same clone as 16019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16019-1-AP (same clone as 16019-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DBR1 antibody (16019-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16019-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16019-1-PBS cible DBR1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DBR1 Protéine recombinante Ag8830
• Nom complet: debranching enzyme homolog 1 (S. cerevisiae)
• Masse moléculaire calculée: 544 aa, 62 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC009472
• Symbole du gène: DBR1
• Identification du gène (NCBI): 51163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.

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