Anticorps Polyclonal de lapin anti-DBT

DBT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 12451-1-AP

Synonymes

BCKDHE2, BCKAD-E2, BCKADE2, BCATE2, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis

---

Galerie de données de validation

• 

  WB analysis using 12451-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12451-1-AP (DBT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12451-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12451-1-AP (DBT antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 12451-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12451-1-AP (DBT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 12451-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12451-1-AP (DBT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12451-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DBT antibody (12451-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules NCI-H1299, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1500-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12451-1-AP cible DBT dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DBT Protéine recombinante Ag3101
• Nom complet: dihydrolipoamide branched chain transacylase E2
• Masse moléculaire calculée: 482 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC016675
• Symbole du gène: DBT
• Identification du gène (NCBI): 1629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nat Metab; Branched-chain amino acid catabolism in muscle affects systemic BCAA levels but not insulin resistance; Authors - Megan C Blair
mouse	WB,IP	J Nutr; Pyruvate Kinase M2 Supports Muscle Progenitor Cell Proliferation but Is Dispensable for Skeletal Muscle Regeneration after Injury.; Authors - Jamie E Blum
human,mouse	WB	Heliyon; Hyperactivation of mTOR and AKT in a cardiac hypertrophy animal model of Friedreich ataxia; Authors - Wing-Hang Tong
mouse	WB	Cell Metab; Extra-cardiac BCAA catabolism lowers blood pressure and protects from heart failure; Authors - Danielle Murashige
human	WB	Front Immunol; Overexpression of DBT suppresses the aggressiveness of renal clear cell carcinoma and correlates with immune infiltration; Authors - Chiyu Zhang
mouse	WB	J Thromb Haemost; Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling; Authors - Haojie Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Maria (Verified Customer) (08-15-2021)

DBT in human primary fibroblasts. 10 ug of total protein. 4-15% TGX gel. Blocking 3% BSA in PBST (0.1% Tween). 1:1000 primary ab O/N incubation at 4ºC. 1:5000 secondary ab HRP conj. 1h RT in BSA 3% in PBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary fibroblasts

DBT Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:12451-1-AP)

{{ptg:RelatedPrimaryAntibodies}}