Anticorps Recombinant de lapin anti-DCC

DCC Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250085A11

N° de cat : 85787-4-PBS

Synonymes

Colorectal cancer suppressor, CRC18, CRCR1, IGDCC1

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Galerie de données de validation

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  WB analysis of A-673 using 85787-4-RR (same clone as 85787-4-PBS)

  A-673 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85787-4-PBS in a different storage buffer formulation.

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  WB analysis of U266 using 85787-4-RR (same clone as 85787-4-PBS)

  U266 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85787-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02122-3

  Sandwich ELISA standard curve of MP02122-3, Human DCC Recombinant Matched Antibody Pair - PBS only. 85787-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2937. 85787-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP02122-3

  The mean DCC concentration was determined to be 11.21 ng/mL in SH-SY5Y cell extract based on a 1.00 mg/mL extract load and 210.63 ng/mL in mouse brain tissue extract based on a 6.60 mg/mL extract load.

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Informations sur le produit

85787-4-PBS cible DCC dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DCC Protéine recombinante Eg2937
• Nom complet: deleted in colorectal carcinoma
• Masse moléculaire calculée: 158 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: NM_005215.3
• Symbole du gène: DCC
• Identification du gène (NCBI): 1630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. DCC gene is a tumor suppressor gene. This antibody raised against a synthesized polypeptide of human DCC protein recognizes endogenous DCC with an apparent molecular weight of 180 kDa. As the predicted molecular mass of DCC is 158 kDa, the slower mobility is likely attributable to post-translational modification, perhaps by glycosylation at potential N-linked glycosylation sites (PMID: 7926722; 8044801).

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