Anticorps Polyclonal de lapin anti-DCDC2

• Phare
• Validé par KD/KO

DCDC2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 26978-1-AP

Synonymes

DCDC2A, Doublecortin domain-containing protein 2, Protein RU2S, RU2, RU2S

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Galerie de données de validation

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  WB analysis of mouse placenta using 26978-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 26978-1-AP (DCDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26978-1-AP

  WB result of DCDC2 antibody (26978-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCDC2 transfected HepG2 cells.

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Applications testées

• Résultats positifs en WB: tissu placentaire de souris, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

26978-1-AP cible DCDC2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCDC2 Protéine recombinante Ag25577
• Nom complet: doublecortin domain containing 2
• Masse moléculaire calculée: 476 aa, 53 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC050704
• Symbole du gène: DCDC2
• Identification du gène (NCBI): 51473
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DCDC2 (Doublecortin domain-containing protein 2), also known as KIAA1154. DCDC2 is expressed in the central nervous system of adults and fetuses, especially in the olfactory cortex, inferior temporal cortex, medial temporal cortex, hypothalamus, amygdala and hippocampus. DCDCDC2 protein contains two dicortical protein domains that bind to microtubules and enhance microtubule polymerization, which may play a role in neuronal migration and affect the signal transduction of primary cilia. In addition, DCDC2 also plays a role in inhibiting classical WNT signaling. DCDC2 may affect the development and function of the brain by affecting the migration of neurons and the signal conduction of primary cilia, and then it is related to reading ability (PMID: 21457949). The molecular weight of DCDC2 is 52 kDa, it also has another isoform with a molecular weight of 25 kDa.

Publications

Species	Application	Title
mouse	WB	Physiol Genomics; Polycystin-2-dependent transcriptome reveals early response of autosomal dominant polycystic kidney disease; Authors - Hyun Jun Jung
mouse,human	WB,IHC,IF	Sci Rep; DCDC2 inhibits hepatic stellate cell activation and ameliorates CCl4-induced liver fibrosis by suppressing Wnt/β-catenin signaling; Authors - Qing-Qing Liu
rat	IF	Front Immunol; TAK1 inhibition mitigates intracerebral hemorrhage-induced brain injury through reduction of oxidative stress and neuronal pyroptosis via the NRF2 signaling pathway; Authors - Jing Zhao

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