Anticorps Polyclonal de lapin anti-DCI

DCI Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11535-1-PBS

Synonymes

3,2-trans-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, EC:5.3.3.8

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Galerie de données de validation

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  WB analysis using 11535-1-AP (same clone as 11535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis using 11535-1-AP (same clone as 11535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis of human lung using 11535-1-AP (same clone as 11535-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11535-1-AP (same clone as 11535-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 11535-1-AP (same clone as 11535-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 11535-1-AP (same clone as 11535-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11535-1-AP (same clone as 11535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11535-1-AP (DCI antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 11535-1-AP (same clone as 11535-1-PBS)

  Immunofluorescent analysis of BxPC-3 cells using 11535-1-AP (DCI antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 11535-1-AP (same clone as 11535-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DCI antibody (11535-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11535-1-PBS cible DCI dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCI Protéine recombinante Ag2094
• Nom complet: dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
• Masse moléculaire calculée: 302 aa, 33 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC020228
• Symbole du gène: DCI
• Identification du gène (NCBI): 1632
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).

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