Anticorps Polyclonal de lapin anti-DCLK2

DCLK2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25450-1-PBS

Synonymes

DCAMKL2, CLICK-II, CLICK2, CLICK II, CL2

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Galerie de données de validation

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  WB analysis using 25450-1-AP (same clone as 25450-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25450-1-AP (DCLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25450-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 25450-1-AP (same clone as 25450-1-PBS)

  IP result of anti-DCLK2 (IP:25450-1-AP, 4ug; Detection:25450-1-AP 1:3000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 25450-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 25450-1-AP (same clone as 25450-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCLK2 antibody (25450-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 25450-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25450-1-PBS cible DCLK2 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCLK2 Protéine recombinante Ag22142
• Nom complet: doublecortin-like kinase 2
• Masse moléculaire calculée: 766 aa, 84 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC032726
• Symbole du gène: DCLK2
• Identification du gène (NCBI): 166614
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DCLK2 belongs to the protein kinase superfamily, contains two N-terminal doublecortin domains, a C-terminal serine/threonine protein kinase domain, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains. DCLK2 is expressed in the brain, heart and eyes (PMID: 18075264).

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