Anticorps Recombinant de lapin anti-DCP1A

DCP1A Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242553F2

N° de cat : 85047-5-PBS

Synonymes

EC:3.6.1.62, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360

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Galerie de données de validation

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  WB analysis using 85047-5-RR (same clone as 85047-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85047-5-RR (DCP1A antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85047-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85047-5-RR (same clone as 85047-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85047-5-RR (DCP1A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85047-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85047-5-RR (same clone as 85047-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85047-5-RR (DCP1A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85047-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85047-5-RR (same clone as 85047-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85047-5-RR (DCP1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85047-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85047-5-RR (same clone as 85047-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85047-5-RR (DCP1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85047-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85047-5-RR (same clone as 85047-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DCP1A antibody (85047-5-RR, Clone: 242553F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85047-5-PBS in a different storage buffer formulation.

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Informations sur le produit

85047-5-PBS cible DCP1A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DCP1A Protéine recombinante Ag18027
• Nom complet: DCP1 decapping enzyme homolog A (S. cerevisiae)
• Masse moléculaire calculée: 582 aa, 63 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC007439
• Symbole du gène: DCP1A
• Identification du gène (NCBI): 55802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DCP1 decapping enzyme homolog A (S. cerevisiae), known as mRNA-decapping enzyme 1A (DCP1A),is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. This antibody specifically react with the 70kd DCP1A protein.

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