Anticorps Monoclonal anti-DCPS

DCPS Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B2E4

N° de cat : 67311-1-PBS

Synonymes

DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015

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Galerie de données de validation

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  WB analysis using 67311-1-Ig (same clone as 67311-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67311-1-Ig (same clone as 67311-1-PBS)

  WB result of DCPS antibody (67311-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCPS transfected HeLa cells. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 67311-1-Ig (same clone as 67311-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67311-1-Ig (same clone as 67311-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67311-1-Ig (same clone as 67311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67311-1-Ig (same clone as 67311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67311-1-Ig (same clone as 67311-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using DCPS antibody (67311-1-Ig, Clone: 1B2E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67311-1-PBS cible DCPS dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DCPS Protéine recombinante Ag8872
• Nom complet: decapping enzyme, scavenger
• Masse moléculaire calculée: 337 aa, 39 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC014532
• Symbole du gène: DCPS
• Identification du gène (NCBI): 28960
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).

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