Anticorps Monoclonal anti-DCT

DCT Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B3D4

N° de cat : 68114-1-PBS

Synonymes

1B3D4, DT, EC:5.3.3.12, L-dopachrome Delta-isomerase, L-dopachrome tautomerase

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Galerie de données de validation

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  WB analysis of A375 using 68114-1-Ig (same clone as 68114-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 68114-1-Ig (same clone as 68114-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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  WB analysis of pig skin using 68114-1-Ig (same clone as 68114-1-PBS)

  pig skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 68114-1-Ig (same clone as 68114-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 68114-1-Ig (same clone as 68114-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68114-1-Ig (same clone as 68114-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (68114-1-Ig, Clone: 1B3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68114-1-PBS cible DCT dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DCT Protéine recombinante Ag26165
• Nom complet: dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
• Masse moléculaire calculée: 519 aa, 59 kDa
• Poids moléculaire observé: 55-63 kDa
• Numéro d’acquisition GenBank: BC028311
• Symbole du gène: DCT
• Identification du gène (NCBI): 1638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

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