Anticorps Monoclonal anti-DCTD

DCTD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D2G12

N° de cat : 68357-1-PBS

Synonymes

dCMP deaminase, DCTD, Deoxycytidylate deaminase

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Galerie de données de validation

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  WB analysis using 68357-1-Ig (same clone as 68357-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68357-1-Ig (DCTD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68357-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68357-1-Ig (same clone as 68357-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DCTD antibody (68357-1-Ig, Clone: 3D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68357-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68357-1-PBS cible DCTD dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DCTD Protéine recombinante Ag10245
• Nom complet: dCMP deaminase
• Masse moléculaire calculée: 178 aa, 20 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC001286
• Symbole du gène: DCTD
• Identification du gène (NCBI): 1635
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).

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