Anticorps Polyclonal de lapin anti-DCTN6

• Phare
• Validé par KD/KO

DCTN6 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 16947-1-AP

Synonymes

WS 3, Protein WS-3, Protein WS 3, Dynactin subunit p27, Dynactin subunit 6

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Galerie de données de validation

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  WB analysis using 16947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16947-1-AP (DCTN6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16947-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16947-1-AP (DCTN6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 16947-1-AP

  IP result of anti-DCTN6 (IP:16947-1-AP, 4ug; Detection:16947-1-AP 1:500) with mouse testis tissue lysate 4800ug.

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  IHC staining of mouse testis using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16947-1-AP (DCTN6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16947-1-AP (DCTN6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16947-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16947-1-AP (DCTN6 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, tissu pulmonaire de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire de souris, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:600-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

16947-1-AP cible DCTN6 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DCTN6 Protéine recombinante Ag10561
• Nom complet: dynactin 6
• Masse moléculaire calculée: 190 aa, 21 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC013175
• Symbole du gène: DCTN6
• Identification du gène (NCBI): 10671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
Monkey	WB	EMBO J; Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.; Authors - Yeh Ting-Yu TY ; KD Validated
human	WB	Curr Biol; An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.; Authors - Florencia di Pietro
human	WB	Mol Biol Cell; Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles.; Authors - Samantha L Cavolo
mouse	WB,IF,IP	Neurobiol Aging; Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains.; Authors - Md Golam Sharoar ; KD Validated
human,mouse	WB	Cell Rep; The Chlamydia effector Dre1 binds dynactin to reposition host organelles during infection; Authors - Jessica Sherry

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zakia (Verified Customer) (09-23-2025)

The DCTN6 (Rb/IgG) worked as expected. we observed a clear reduction in dctn6 expression, and interestingly, a decrease in DCTN5 levels was also noted. Overall ,the antibody performed reliable and produced consistent results.

• Applications: Western Blot, Protein purification
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human post mortem Tissue

DCTN6 Antibody Western Blot,Protein purification validation (1:1000 dilution) in Human post mortem Tissue (Cat no:16947-1-AP)

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