Anticorps Recombinant de lapin anti-DCX

DCX Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241985H1

N° de cat : 84595-4-PBS

Synonymes

Lis-X, Lissencephalin-X, Doublin, doublecortin, 241985H1

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Galerie de données de validation

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  WB analysis of fetal human brain using 84595-4-RR (same clone as 84595-4-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 84595-4-RR (same clone as 84595-4-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84595-4-RR (DCX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 84595-4-RR (same clone as 84595-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84595-4-RR (DCX antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84595-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84595-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84595-4-RR against Human DCX were performed. The affinity constant is 1.23 nM.

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Informations sur le produit

84595-4-PBS cible DCX dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DCX Protéine recombinante Ag4945
• Nom complet: doublecortin
• Masse moléculaire calculée: 402 aa, 45 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC027925
• Symbole du gène: DCX
• Identification du gène (NCBI): 1641
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

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