Anticorps Polyclonal de lapin anti-DDAH2

• Phare
• Validé par KD/KO

DDAH2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14966-1-PBS

Synonymes

DDAH, DDAH 2, DDAH-2, DDAHII, Dimethylargininase 2

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Galerie de données de validation

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  WB analysis using 14966-1-AP (same clone as 14966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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  WB analysis using 14966-1-AP (same clone as 14966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 14966-1-AP (same clone as 14966-1-PBS)

  WB result of DDAH2 antibody (14966-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDAH2 transfected MCF-7 cells. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14966-1-AP (same clone as 14966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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  IP experiment of COLO 320 using 14966-1-AP (same clone as 14966-1-PBS)

  IP result of anti-DDAH2 (IP:14966-1-AP, 3ug; Detection:14966-1-AP 1:500) with COLO 320 cells lysate 2000ug. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14966-1-PBS cible DDAH2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDAH2 Protéine recombinante Ag6831
• Nom complet: dimethylarginine dimethylaminohydrolase 2
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC001435
• Symbole du gène: DDAH2
• Identification du gène (NCBI): 23564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.(PMID:21898353).

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