Anticorps Monoclonal anti-DDAH2

DDAH2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A10G3

N° de cat : 68154-1-PBS

Synonymes

Dimethylargininase 2, DDAHII, DDAH-2, DDAH 2, DDAH

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Galerie de données de validation

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  WB analysis using 68154-1-Ig (same clone as 68154-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse retina using 68154-1-Ig (same clone as 68154-1-PBS)

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

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  WB analysis of rat colon using 68154-1-Ig (same clone as 68154-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50417-2

  Cytometric bead array standard curve of MP50417-2, DDAH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68154-4-PBS. Detection antibody: 68154-1-PBS. Standard:Ag7127. Range: 0.391-50 ng/mL.

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Informations sur le produit

68154-1-PBS cible DDAH2 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DDAH2 Protéine recombinante Ag7127
• Nom complet: dimethylarginine dimethylaminohydrolase 2
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC001435
• Symbole du gène: DDAH2
• Identification du gène (NCBI): 23564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.

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