Anticorps Monoclonal anti-DDB1

• Phare
• Validé par KD/KO

DDB1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

4F6C6

N° de cat : 66010-1-Ig

Synonymes

DDB p127 subunit, DDB1, DDBA, DNA damage binding protein 1, DNA damage binding protein a, HBV X associated protein 1, UV damaged DNA binding factor, UV DDB 1, UV DDB1, XAP 1, XAP1, XPCE, XPE, XPE BF, XPE binding factor

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Galerie de données de validation

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  WB analysis using 66010-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of A549 using 66010-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66010-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig (DDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 66010-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66010-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66010-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66010-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66010-1-Ig (DDB1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66010-1-Ig

  Immunofluorescent analysis of fixed MCF-7 cells using 66010-1-Ig (DDB1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7
• Résultats positifs en IHC: tissu d'appendicite humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 3 publications below

Informations sur le produit

66010-1-Ig cible DDB1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DDB1 Protéine recombinante Ag17903
• Nom complet: damage-specific DNA binding protein 1, 127kDa
• Masse moléculaire calculée: 1140 aa, 127 kDa
• Poids moléculaire observé: 127 kDa
• Numéro d’acquisition GenBank: BC011686
• Symbole du gène: DDB1
• Identification du gène (NCBI): 1642
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.

Publications

Species	Application	Title
human	WB	J Clin Invest; Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.; Authors - Yajuan Zhang ; KD Validated
human	WB	J Virol; Degradation of SAMHD1 by Vpx is Independent of Uncoating.; Authors - Paula Jáuregui ; KD Validated
human	WB	J Virol; Human herpesvirus 6A U4 inhibits proteasomal degradation of amyloid precursor protein.; Authors - Tian Tang ; KD Validated

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