Anticorps Recombinant de lapin anti-DYKDDDDK tag
DYKDDDDK tag Recombinant Antibody for IF, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Protéine recombinante et plus (3)
Applications
RIP, IP, IF, FC, CoIP, ELISA
Conjugaison
Non conjugué
CloneNo.
4K14
N° de cat : 80010-1-RR
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IP | cellules HEK-293 transfectées |
| Résultats positifs en IF | cellules HEK-293 transfectées, cellules HEK-293 transfectées |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| IF | See 8 publications below |
| IP | See 24 publications below |
| FC | See 1 publications below |
| CoIP | See 8 publications below |
| RIP | See 1 publications below |
Informations sur le produit
80010-1-RR cible DYKDDDDK tag dans les applications de RIP, IP, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | Humain, singe, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Recombinant |
| Type | Anticorps |
| Immunogène | DYKDDDDK tag Protéine recombinante Ag2329 |
| Nom complet | DYKDDDDK tag |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for DYKDDDDK tag antibody 80010-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
PLoS Biol CMPK2 is a host restriction factor that inhibits infection of multiple coronaviruses in a cell-intrinsic manner | ||
Cancer Lett N-myristoyltransferase-1 deficiency blocks myristoylation of LAMTOR1 and inhibits bladder cancer progression. | ||
Cancer Lett ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xin (Verified Customer) (04-17-2022) | It is good to detect flag-tagged protein expressed in hek293 cells, although efficiency is not so high as 20543-1-AP.
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