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Anticorps Polyclonal de lapin anti-DDI2

DDI2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25377-1-PBS

Synonymes

Protein DDI1 homolog 2

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using DDI2 Polyclonal antibody (25377-1-AP)

    WB analysis using 25377-1-AP (same clone as 25377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25377-1-AP (DDI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25377-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDI2 Polyclonal antibody (25377-1-AP)

    IF Staining of HepG2 using 25377-1-AP (same clone as 25377-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDI2 antibody (25377-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25377-1-PBS in a different storage buffer formulation.

Informations sur le produit

25377-1-PBS cible DDI2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène DDI2 Protéine recombinante Ag21953
Nom complet DDI1, DNA-damage inducible 1, homolog 2 (S. cerevisiae)
Masse moléculaire calculée 399 aa, 45 kDa
Poids moléculaire observé 45-50 kDa
Numéro d’acquisition GenBankBC006011
Symbole du gène DDI2
Identification du gène (NCBI) 84301
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDI2 contains 399 amino acids and contains an N-terminal ubiquitin-like (UBL) domain and a highly conserved retroviral protease-like (RVP) domain. DDI2 is an aspartic endoprotease that cleaves ubiquitylated target proteins to assist in their processing by the ubiquitin-proteasome system (UPS), especially when the UPS is inhibited(PMID: 32521225).

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