Anticorps Polyclonal de lapin anti-DDR1

• Phare
• Validé par KD/KO

DDR1 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 10536-1-AP

Synonymes

DDR, Cell adhesion kinase, CD167a, CD167 antigen-like family member A, CD167

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Galerie de données de validation

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  IHC staining of human colon cancer using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10536-1-AP (DDR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10536-1-AP (DDR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10536-1-AP (DDR1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10536-1-AP (DDR1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10536-1-AP (DDR1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10536-1-AP (DDR1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10536-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10536-1-AP (DDR1 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu d'estomac humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10536-1-AP cible DDR1 dans les applications de IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDR1 Protéine recombinante Ag0794
• Nom complet: discoidin domain receptor tyrosine kinase 1
• Masse moléculaire calculée: 97 kDa
• Numéro d’acquisition GenBank: BC008716
• Symbole du gène: DDR1
• Identification du gène (NCBI): 780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDR1, or Discoidin Domain Receptor 1, is a member of the receptor tyrosine kinase (RTK) family, which plays a significant role in various cellular processes including cell proliferation, adhesion, migration, and extracellular matrix (ECM) remodeling. DDR1 is known for its unique discoidin domain that allows it to bind specifically to certain collagens, initiating downstream signaling pathways that can lead to cell transformation and tumor progression. Abnormal activation of DDR1 is closely associated with the development of various solid tumors, and it has been shown that DDR1 can prevent immune cells from infiltrating triple-negative breast cancer (TNBC) and eliminate tumor cells.

Publications

Species	Application	Title
human,mouse	IF,CoIP	Nat Commun; Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation; Authors - Jiayu Liu ; KD Validated
human	IHC,IF	Bioact Mater; Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.; Authors - Jin Wang ; KD Validated
human	IHC	J Exp Clin Cancer Res; High expression of DDR1 is associated with the poor prognosis in Chinese patients with pancreatic ductal adenocarcinoma.; Authors - Yanmiao Huo
human		Exp Cell Res; Downregulation of CYRI-B promotes migration, invasion and EMT by activating the Rac1-STAT3 pathway in gastric cancer; Authors - Guangyao Liu

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