Anticorps Polyclonal de lapin anti-DDRGK1

• Phare
• Validé par KD/KO

DDRGK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21445-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 21445-1-AP (same clone as 21445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21445-1-AP (DDRGK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 21445-1-AP (same clone as 21445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21445-1-AP (same clone as 21445-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDRGK1 antibody (21445-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21445-1-PBS cible DDRGK1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDRGK1 Protéine recombinante Ag13742
• Nom complet: DDRGK domain containing 1
• Masse moléculaire calculée: 314 aa, 36 kDa
• Poids moléculaire observé: 36-46 kDa
• Numéro d’acquisition GenBank: BC000643
• Symbole du gène: DDRGK1
• Identification du gène (NCBI): 65992
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.

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