Anticorps Polyclonal de lapin anti-DDX10

• Phare
• Validé par KD/KO

DDX10 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17857-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of SMMC-7721 using 17857-1-AP (same clone as 17857-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 17857-1-AP (same clone as 17857-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

17857-1-PBS cible DDX10 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX10 Protéine recombinante Ag12317
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
• Masse moléculaire calculée: 875 aa, 101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC091521
• Symbole du gène: DDX10
• Identification du gène (NCBI): 1662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).

{{ptg:RelatedPrimaryAntibodies}}