Anticorps Polyclonal de lapin anti-DDX17

• Phare
• Validé par KD/KO

DDX17 Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-19910

Synonymes

DDX17, DEAD box protein 17, DEAD box protein p72, DEAD box protein p82, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX17, RNA-dependent helicase p72, DDX 17

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Galerie de données de validation

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  IF Staining of HEK-293 using CL488-19910

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 DDX17 antibody (CL488-19910) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HEK-293 using CL488-19910

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated DDX17 Polyclonal antibody (CL488-19910)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules HEK-293,
• Résultats positifs en FC (Intra): cellules HEK-293
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-19910 cible DDX17 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX17 Protéine recombinante Ag13723
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
• Masse moléculaire calculée: 729 aa, 80 kDa
• Poids moléculaire observé: 72-80 kDa
• Numéro d’acquisition GenBank: BC000595
• Symbole du gène: DDX17
• Identification du gène (NCBI): 10521
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX17, also named as DEAD box protein p72 and DEAD box protein p82, is a 729 amino acid protein, which belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. DDX17 is widely expressed. Levels tend to increase during colon cancer progression, from very low in benign hyperplastic polyps to very high in tubular and villous adenomas. DDX17 as an RNA helicase unwinds RNA and alters RNA structures through ATP binding and hydrolysis. DDX17 is involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, ribosomal RNA processing and miRNA processing, as well as transcription regulation. It Regulates the alternative splicing of exons exhibiting specific features. identification and characterisation of p72, a novel human nuclear DEAD box protein, which shows a striking homology to p68. a predicted molecular mass of DDX17 is 72 kDa, but endogenous DDX17 migrates aberrantly at 79 kDa on SDS-PAGE (PMID: 8871553).

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