Anticorps Recombinant de lapin anti-DDX18

DDX18 Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250053A4

N° de cat : 85789-2-PBS

Synonymes

ATP-dependent RNA helicase DDX18, cPERP-D, DEAD box protein 18, EC:3.6.4.13, MrDb

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Galerie de données de validation

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  WB analysis using 85789-2-RR (same clone as 85789-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85789-2-RR (DDX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85789-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02107-2

  The mean DDX18 concentration was determined to be 249.8 ng/mL in HEK-293T cell extract based on a 2.8 mg/mL extract load, 244.1 ng/mL in K-562 cell extract based on a 2.5 mg/mL extract load and 166.4 ng/mL in HepG2 cell extract based on a 1.8 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02107-2

  Sandwich ELISA standard curve of MP02107-2, Human DDX18 Recombinant Matched Antibody Pair - PBS only. 85789-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27457. 85789-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  IF Staining of HepG2 using 85789-2-RR (same clone as 85789-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX18 antibody (85789-2-RR, Clone: 250053A4 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85789-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85789-2-RR (same clone as 85789-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX18 antibody (85789-2-RR, Clone: 250053A4 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85789-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85789-2-PBS

  Biolayer interferometry (BLl) kinetic assays of 85789-2-PBS against Human DDX18 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85789-2-PBS cible DDX18 dans les applications de WB, IF/ICC, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DDX18 Protéine recombinante Ag27457
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC001238
• Symbole du gène: DDX18
• Identification du gène (NCBI): 8886
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDX18 (DEAD-box helicase 18) is a protein belonging to the DEAD-box deconjugating enzyme family that is widely involved in RNA metabolism. It plays a key role in the assembly of the ribosomal small subunit (SSU) and ensures efficient intracellular protein synthesis. DDX18 unwinds RNA double strands through its deconjugating enzyme activity, which is essential for the proper processing and functional execution of RNA molecules. In addition, DDX18 is a multifunctional RNA deconjugating enzyme involved in a variety of signaling pathways, playing a key role not only in ribosome assembly and RNA metabolism, but also in the regulation of a variety of biological processes such as cell cycle, genome stability and stem cell pluripotency.

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