Anticorps Recombinant de lapin anti-DDX18

DDX18 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250053A4

N° de cat : 85789-2-RR

Synonymes

ATP-dependent RNA helicase DDX18, cPERP-D, DEAD box protein 18, EC:3.6.4.13, MrDb

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Galerie de données de validation

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  WB analysis using 85789-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85789-2-RR (DDX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85789-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX18 antibody (85789-2-RR, Clone: 250053A4 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1250-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85789-2-RR cible DDX18 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DDX18 Protéine recombinante Ag27457
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC001238
• Symbole du gène: DDX18
• Identification du gène (NCBI): 8886
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX18 (DEAD-box helicase 18) is a protein belonging to the DEAD-box deconjugating enzyme family that is widely involved in RNA metabolism. It plays a key role in the assembly of the ribosomal small subunit (SSU) and ensures efficient intracellular protein synthesis. DDX18 unwinds RNA double strands through its deconjugating enzyme activity, which is essential for the proper processing and functional execution of RNA molecules. In addition, DDX18 is a multifunctional RNA deconjugating enzyme involved in a variety of signaling pathways, playing a key role not only in ribosome assembly and RNA metabolism, but also in the regulation of a variety of biological processes such as cell cycle, genome stability and stem cell pluripotency.

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