Anticorps Monoclonal anti-DDX21

DDX21 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1B9C8

N° de cat : 66925-1-Ig

Synonymes

GUA, Gu alpha, EC:3.6.4.13, DExD-box helicase 21, DEAD box protein 21

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Galerie de données de validation

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  WB analysis using 66925-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66925-1-Ig (DDX21 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 66925-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66925-1-Ig (DDX21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66925-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66925-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66925-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66925-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66925-1-Ig (DDX21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66925-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (66925-1-Ig, Clone: 1B9C8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 66925-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human DDX21 (66925-1-Ig, Clone:1B9C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HL-60, cellules Jurkat, cellules K-562, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66925-1-Ig cible DDX21 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DDX21 Protéine recombinante Ag17850
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• Masse moléculaire calculée: 783 aa, 87 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: BC008071
• Symbole du gène: DDX21
• Identification du gène (NCBI): 9188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing.

Publications

Species	Application	Title
human	WB,IP,IHC	Cell Death Dis; TRIP13 regulates progression of gastric cancer through stabilising the expression of DDX21; Authors - Guanghui Zhang
human		Nat Commun; Virus-modified paraspeckle-like condensates are hubs for viral RNA processing and their formation drives genomic instability; Authors - Katherine L Harper

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