Anticorps Recombinant de lapin anti-DDX21

• Phare
• Validé par KD/KO

DDX21 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5E7

N° de cat : 80932-1-PBS

Synonymes

5E7, DEAD box protein 21, DExD-box helicase 21, EC:3.6.4.13, Gu alpha

---

Galerie de données de validation

• 

  WB analysis using 80932-1-RR (same clone as 80932-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80932-1-RR (DDX21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 80932-1-RR (same clone as 80932-1-PBS)

  WB result of DDX21 antibody (80932-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX21 transfected HeLa cells. This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 80932-1-RR (same clone as 80932-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX21 antibody (80932-1-RR, Clone: 5E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80932-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80932-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80932-1-RR against Human DDX21 were performed. The affinity constant is 77.7 nM.

---

Informations sur le produit

80932-1-PBS cible DDX21 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DDX21 Protéine recombinante Ag0804
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 87 kDa
• Numéro d’acquisition GenBank: BC008071
• Symbole du gène: DDX21
• Identification du gène (NCBI): 9188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDX21 protein belongs to DEAD box protein family which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). As a putative RNA helicase, DDX21 unwinds double-stranded RNA, folds single-stranded RNA and is involved in process including ribosomal RNA biogeneis, RNA editing and general transcription. Interaction of DDX21 and c-Jun was reported in ribosomal RNA processing. DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa.

{{ptg:RelatedPrimaryAntibodies}}