Anticorps Polyclonal de lapin anti-DDX24

• Phare
• Validé par KD/KO

DDX24 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15769-1-PBS

Synonymes

EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24

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Galerie de données de validation

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  WB analysis using 15769-1-AP (same clone as 15769-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 15769-1-AP (DDX24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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  WB analysis using 15769-1-AP (same clone as 15769-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15769-1-AP (DDX24 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 15769-1-AP (same clone as 15769-1-PBS)

  WB result of DDX24 antibody (15769-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX24 transfected A549 cells. This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 15769-1-AP (same clone as 15769-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15769-1-AP (DDX24 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15769-1-AP (same clone as 15769-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX24 antibody (15769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15769-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15769-1-PBS cible DDX24 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX24 Protéine recombinante Ag8407
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC008847
• Symbole du gène: DDX24
• Identification du gène (NCBI): 57062
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.

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